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- W2185099110 abstract "Black layer has been associated with a severe decline in the quality of turf on putting greens. It was suggested that the black layer results from dissimilatory sulfate (SO 4 2– ) reduction. This study was done to determine if SO 4 2– reduction occurs in an existing black layer. Radioactive 35 SO 4 2– was used to calculate the rate of SO 4 2– reduction in intact soil cores taken from an existing black layer in a `Penncross' creeping bentgrass ( Agrostis palustris Huds. `Penncross') putting green. When 10 –3 M 35 SO 4 2– with a specific activity of 1.554 × 10 5 Bq·mg –1 SO 4 2– was injected into a core it reduced to sulfide ( 35 S 2– ) at a mean rate of 7.1 nmol sulfur (S)/cm 3 soil/d. Injecting azide (N 3 – ) or molybdate (MoO 4 2– ) at 10% w/v with the label reduced the rate of SO 4 2– reduction to 0.03 and 0.01 nmol S/cm 3 soil/d, respectively. The effect of N 3 – confirmed that reduction of SO 4 2– was biological, while the effect of MoO 4 2– confirmed that the entities responsible for the reductive cycling were sulfate-reducing bacteria (SRBs). This was the first proof that biological reduction of SO 4 2– produces S 2– in a black layer from a creeping bentgrass putting green. It was concluded that the respiration of indigenous SRBs was linked to development of this black layer. Thus, a key to successfully controlling black layer in putting greens must involve regulating the respiratory activities of SRBs." @default.
- W2185099110 created "2016-06-24" @default.
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- W2185099110 date "2006-06-01" @default.
- W2185099110 modified "2023-10-17" @default.
- W2185099110 title "Dissimilatory Reduction of Sulfate in Black Layer" @default.
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- W2185099110 doi "https://doi.org/10.21273/hortsci.41.3.815" @default.
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