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• W2185133083 abstract "d-serine is an amino acid that has an important role in the regulation of N-methyl-d-aspartate (NMDA) signalling in the central nervous system. In fact, it acts as an endogenous co-agonist molecule for the NMDA receptor activation. Since an abnormal body level of d-amino acids is usually associated to different neurological diseases, it appears evident that it is of high interest to develop a fast and specific biosensor for the determination of d-serine. In this work, we report on the design and development of a surface acoustic wave (SAW) biosensor to detect the level of d-serine. The SAW-biosensor is based on the utilization of an inactivated form of the d-serine dehydratase isolated from Saccharomyces cerevisiae, a biomolecule that is able to specifically bind d-serine. In particular, we produced a PLP-reduced d-serine dehydratase biomolecule that was still able to bind d-serine and not to transform it into the product. The binding event between the “inactivated” d-serine dehydratase and d-serine was monitored by SAW delay-lines." @default.
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• W2185133083 date "2016-04-01" @default.
• W2185133083 modified "2023-10-18" @default.
• W2185133083 title "A Shear horizontal surface acoustic wave biosensor for a rapid and specific detection of d-serine" @default.
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• W2185133083 doi "https://doi.org/10.1016/j.snb.2015.11.099" @default.
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