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- W2185143779 abstract "Apoptosis is a form of ‘‘planned cell death’’ and is an essential component of normal tissue differentiation and functional regulation. Neutrophil apoptosis facilitates down regulation of the inflammatory response while minimizing ‘‘by stander’’ injury to normal tissue, and disruption of this process by various diseases may have a significant negative impact on patient recovery. Consequently, neutrophil apoptosis has been the focus of research in many species. However, methods for measuring apoptosis have not been evaluated in the horse. The goal of this study was to adapt previously reported methods for inducing and measuring both neutrophil apoptosis and necrosis in non-equine species for use in equine peripheral blood neutrophils. To achieve this goal the experiment was divided into three parts: 1. Induce apoptosis and necrosis in equine peripheral blood neutrophils using previously used known inducers and examine the relationship between exposure time and percentage of affected cells; 2. Measure percentage of apoptosis and necrosis using three methods of detection: a) Annexin-V Fitc PI assay, b) Homogenous caspase 3/7 assay and c) Light microscopy and; 3. Compare the results between the three methods of apoptosis detection to determine if results are comparable" @default.
- W2185143779 created "2016-06-24" @default.
- W2185143779 creator A5061129701 @default.
- W2185143779 date "2007-06-15" @default.
- W2185143779 modified "2023-09-27" @default.
- W2185143779 title "Methods to Detect Apoptosis in Equine Peripheral Blood Neutrophils from Normal Healthy Adult Horses" @default.
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- W2185143779 hasPublicationYear "2007" @default.
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