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- W2185378098 abstract "Ribosome display has proven to be a powerful in vitro selection and evolution method for generating high-affi nity binders from libraries of folded proteins. It has been successfully applied to single-chain Fv fragments of antibodies and alternative scaffolds, such as D esigned A nkyrin R epeat P roteins (DARPins). High-affi nity binders with new target specifi city can be obtained from highly diverse DARPin libraries in only a few selection rounds. In this protocol, the selection from the library and the process of affi nity maturation and off-rate selection are explained in detail." @default.
- W2185378098 created "2016-06-24" @default.
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- W2185378098 date "2012-01-01" @default.
- W2185378098 modified "2023-09-27" @default.
- W2185378098 title "Rapid Selection of High-Affi nity Binders Using Ribosome Display" @default.
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