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- W2185394757 abstract "Background: Nucleolin (NU) can be found in the nucleus in aggregates and in a diffused form. The expression and distribution of these two intranuclear pools of nucleolin was compared in estrogen receptor (ER)-positive and -negative invasive ductal breast carcinomas in relation to cell cycle phases. Materials and Methods: Using a laser scanning cytometer NU was measured within the whole nucleus, within nucleolin aggregates (NUA) and within the NUA-free karyoplasm. The G1-, S- and G2M-phase cell subpopulations were distinguished on DNA histograms. Results: The nuclei of ER-positive and ER-negative breast carcinomas in the G2M- phase differed in the expression of NU in the NUA and in the NUA-free karyoplasm, whereas in the S-phase they only differed in the NUA-free karyoplasm expression. In ER-positive carcinomas NU levels rose from G1 to G2M in both the NUA and the NUA-free karyoplasm, whereas in ER-negative carcinomas the NU levels increased only between G1/S in the NUA and between S/G2M in the NUA-free karyoplasm. Conclusion: There are significant differences in nucleolin expression and localization during cell cycle progression depending on ER status." @default.
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- W2185394757 date "2007-11-01" @default.
- W2185394757 modified "2023-09-26" @default.
- W2185394757 title "Intranuclear nucleolin distribution during cell cycle progression in human invasive ductal breast carcinomas in relation to estrogen receptor status." @default.
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