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• W2185398812 abstract "Currently, the use of ectoparasiticides on dogs to control ticks and fleas is inevitable, and the safety data for dogs, dog owners, and veterinary personnel are scarce. This investigation was therefore undertaken with two objectives: (1) to determine the residue of fipronil, s-methoprene, and amitraz in dog blood, and (2) to determine the transferable residues of these insecticides to gloves worn while petting experimental household dogs after the topical application of Certifect ® . Certifect ® (4.28 mL) contains 9.8% fipronil, 8.8% s-methoprene, and 22.1% amitraz. With a combination of these three insecticides, Certifect ® kills ticks, fleas and chewing lice of all stages. All three insecticides produce their toxicity by different mechanisms. The blood samples (approximately 3-5 mL from each dog) were collected into EDTA tubes. Gloves were worn for 5 min while petting individual dogs and then collected for pesticide residue analysis. Blood and glove samples were extracted in methylene chloride/petroleum ether (1:1), and the extracts were assayed for residues of fipronil, s-methoprene, and amitraz using GC/MS. Blood analysis revealed the presence of only amitraz (0.42±0.16 µg/ mL) 48 hr post-Certifect ® application. In gloves, significant residues of fipronil, s-methoprene, and amitraz were detected after 24 hr, with maximum transferable residue at 72 hr (432.16±79.18; 301.84±52.51; and 398.37±112.19 µg/g, respectively). After day 7, the concentrations of these insecticides in glove extracts were about 100 µg/g glove wt. While fipronil and s-methoprene were detected until day 28 (0.87±0.55 and 2.66±1.22 µg/g, respectively), the residue of amitraz was detectable only until day 14 (6.01±1.75 µg/g). In conclusion, Certifect ® appears to be safe for dogs and their owners following once a month exposure, but veterinary personnel can be at risk following daily exposure to transferable residues of fipronil, s-methoprene, and amitraz, if not properly protected." @default.
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• W2185398812 date "2014-01-01" @default.
• W2185398812 modified "2023-09-26" @default.
• W2185398812 title "Residue of Fipronil, S-Methoprene, and Amitraz in Dog Blood and in Gloves from Topical Certifect ® Application: Toxicity and Safety Considerations" @default.
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