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- W2185399720 abstract "Prairie turnip (Pediomelum esculentum (Pursh) Rydb. [Fabaceae]), an indigenous legume, is a traditional food for Native Americans of the Great Plains, and an excellent source of protein due to its high nitrogen content. We investigated endophytic diazotrophs as a potential source of nitrogen for the large protein stores contained within the edible tuber. Genomic bacterial DNA was isolated from lower root, bark, tuber, stem, and leaf tissue of a surface sterilized plant. PCR amplification of nifH, a gene that codes for a nitrogenase subunit, was performed to detect nitrogen fixers. Denaturing gradient gel electrophoresis of the nifH PCR product revealed the composition and diversity of these endophytes. The presence of the nifH gene from multiple bacterial strains was confirmed within all examined tissues. The identification of symbiotic nitrogen fixers inside prairie turnip increases the potential of this unique plant to be utilized for novel crop endeavors." @default.
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- W2185399720 date "2014-01-01" @default.
- W2185399720 modified "2023-10-16" @default.
- W2185399720 title "NITROGEN FIXING ENDOPHYTES WITHIN PEDIOMELUM ESCULENTUM" @default.
- W2185399720 hasPublicationYear "2014" @default.
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