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- W2185421214 abstract "This paper presents the pneumatically-driven tensile stimulator for investigating the effect of mechanical strain on the intracellular calcium expression in MG-63 cells (human osteoblast-like bone cell line). An optically transparent micro tensile stimulator array consisting of the deformable diaphragms and the micro fluidic channels was fabricated with a polydimethylsiloxane (PDMS) and a glass substrate. A strain gradient generated by the three-dimensional dome-shape deformation of the circular diaphragm was applied to live cells seeded on the deformable diaphragm of the pneumatic stimulator. During operation, intracellular calcium responses were measured using a laser-scanning microscope. Our new finding from temporal responses of fluorescence intensities was that the period of measured calcium expressions of cells under different strain regions were matched well with the applied signal waveform of the external compressed air, and variations of the calcium expression might be also highly correlated with the magnitude of the strain. The release/uptake of intracellular calcium in the endoplasmic reticulum may be activated and more sensitive to applied strain." @default.
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- W2185421214 date "2011-01-01" @default.
- W2185421214 modified "2023-09-27" @default.
- W2185421214 title "EFFECTS OF MECHANICAL STRAIN ON INTRACELLULAR CALCIUM EXPRESSION IN CELL" @default.
- W2185421214 hasPublicationYear "2011" @default.
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