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• W2185440023 abstract "The present research was conducted to show a new anti-counterfeiting method using Rhodamine B (RB) as a fluorescent labelling on tobacco seeds. Seeds of three tobacco varities, MS Yunyan85 (MS YY85), MS K326 and Honghua Dajintuan (HHDJY) were soaked in RB solutions with different concentrations. The endosperm and embryo of treated seed showed bright red fluorescence under green excitation (530-560 nm) under fluorescent microscope while no red colour was observed in the control seeds. The red fluorescence was also observed in the leaf vein of 5- and7-day-old treated seedlings. So, the bright red fluorescence can be used as an anti-counterfeiting marker for distinguishing true (treated) seed from fake (untreated) seeds. In addition, seed germination and seedling growth stus were investigated after seed soaking. Through principal component analysis, the appropriate fluorescent labelling concentrations were no toxic effect to seed germination and seedling growth were obtained for each tobacco variety as: 0.1-0.5 mg ml-1 for MS YY84, 0.1 mg ml-1 for HHDJY and MS K326. Practically, optimal RB concentration should experimentally be determined for different crops and varieties as seed label. The regression equations of the relation between new principal component and RB concentration were established by the orthogonal polynomials regression analysis based on the combination of qualitative and quantitative traits, which could be used to predict the effects of different RB concentrations on germination of tobacco seeds. Therefore, soaking seed in suitable concentration of RB solution might be an effective fluorescent labelling method for anti-counterfeiting in tobacco seeds." @default.
• W2185440023 created "2016-06-24" @default.
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• W2185440023 date "2013-02-01" @default.
• W2185440023 modified "2023-09-27" @default.
• W2185440023 title "A new effective fluorescent labeling method for anti-counterfeiting of tobacco seed using Rhodamine B" @default.
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