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• W2185471506 abstract "6 Acknowledgements 7 Commonly used Abbreviations 8 Chapter 1: General Introduction 9 The Pacific Oyster Industry in South Australia 9 Stress and Stress Response in Organisms 10 Stress Related Immunosuppression in Pacific Oysters 11 Stress Indicator Criteria for Pacific Oysters 14 The Role of Phenoloxidase in Pacific Oysters 14 Prophenoloxidase-Activating System 15 Measuring Phenoloxidase activity 18 Significance of Study 19 Chapter 2: General Methodology 21 2.1. Experimental Animals 21 2.2 Collection of Oyster Hemolymph 21 2.3 Spectrophotometric Assay of Phenoloxidase (PO) Activity 21 2.3.1 Spectrophotometric Assay using L-3, 4-dihydroxyphenylalanine (L-DOPA) as a Substrate 22 2.3.2 Spectrophotometric Assay using 4-Methoxyphenol (HQ) as a Substrate 23 2.4 Preparation of Oyster Hemolymph for Native PAGE 23 2.4.1 Native Polyacrylamide Gel Electrophoresis (Native PAGE) 24 2.5 Hemolymph Heating 24 2.6 Statistical Analysis 24 Preliminary Methodology 25 2.6 L-DOPA Assay 25 2.6.1 Dilution Series Experiment 25 2.6.2 Second Dilution Series Experiment 27 2.6.3 L-DOPA Substrate Concentration Experiment 28 2.6.4 Centrifugation Experiment 29 2.6.5 Hemolymph Storage Experiment 31 2.7 HQ Assay 32 2.7.1 Dilution Series Experiment 32 2.7.2 HQ Substrate Concentration Experiment 34 2.7.3 Centrifugation Experiment 35 2.8 Tropolone Inhibition Experiment 37 2.8.1 Background 37 2.8.2 L-DOPA assay 38 2.8.3 HQ assay 39" @default.
• W2185471506 created "2016-06-24" @default.
• W2185471506 creator A5077875084 @default.
• W2185471506 date "2008-01-01" @default.
• W2185471506 modified "2023-09-27" @default.
• W2185471506 title "Reduction in Pacific Oyster Mortality by Improving Farming and Processing Technologies in South Australia" @default.
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