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• W2185490003 abstract "A tissue culture experiment was conducted to investigate the effect of some growth regulators and two different ages of in vitro leaves of date palm c.v. sakoty on regeneration process. Leaves were cultured on Murashige and Skoog's medium (MS) supplemented with each or combined with the following growth regulators 4 mg l-1 2,4-D, 40 mg l-1 NAA, 2 mg l-1BA, (2 mg1 -1BA+ 40 mg l-1 NAA), (2mgl-1 BA+ 4 mg l-1 2,4-D) and (2 mg l-1BA+40 mg l-1 NAA+4 mg l-1 2,4-D). The results showed that in vitro young leaf explants treated with 2 mg1 -1 BA was more efficient in inducing direct somatic embryos and subsequent plantlets growth in a short time duration ,i.e.8-10 months. However, the other treatments or the old leaves had no response to induce direct somatic embryos. Also, histological examinations were made during the initiation and development of direct somatic embryos. It was revealed that the proembryos originated from procambial cells, whereas the mesophyll parenchyma and the epidermal cells did not participate in this process. Procambial cells acquired the embryonic competence within 15 days form culturing. The embryonic structures were generated along the vascular strands, which subsequently evolved into somatic embryos. After two months, different developmental stages of somatic embryos were observed, i.e. (pre-globular, globular, early bipolar, bipolar and cotyledonary-shaped). The regenerated embryos were transferred onto both multiplication and rooting media for 8 months to obtained healthy plantlets. Therefore, this protocol provides a simple and rapid way to regenerate date palm via direct embryogenesis. This result obtained herewith was discussed with other results elsewhere which depend on callus formation as an intermediate phase to obtain somatic embryos indirectly." @default.
• W2185490003 created "2016-06-24" @default.
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• W2185490003 date "2014-06-01" @default.
• W2185490003 modified "2023-09-27" @default.
• W2185490003 title "Histological changes on regeneration in vitro culture of date palm (Phoenix dactylifera) leaf explants" @default.
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