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- W2185559547 abstract "An immuno-dipstick test was developed for detection of teliospores of Karnal bunt using crude and DE-52 purified anti-teliospore antibodies. For development of this test, intact teliospores and solubilized teliosporic antigen were coated on nitrocellulose pad of dipsticks. On observation of the nitrocellulose pad under a stereo zoom microscope, the intact teliospores were found stuck on the pad after coating but got washed away with first washing. The positive tests after immunoprocessing of dipsticks indicate that surface antigen may tear off from teliospores and remains bound to nitrocellulose pad. Standardization and optimization of the test was done following antigen concentration kinetics and antibody dilution analysis as well as antibody incubation time. The test was also performed for checking cross-reactivity with other fungal pathogens. DE-52 purified antiserum gave partial reactivity with spores of only Tilletia barclayana and Puccinia recondita. The sensitivity of test was found to be 5 teliospores and 10 ng for solubilized teliosporic antigen." @default.
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- W2185559547 date "2003-03-09" @default.
- W2185559547 modified "2023-09-26" @default.
- W2185559547 title "Design and Optimization of Novel Immuno-dipstick Test for Detection of Teliospores of Kamal Bunt (Tilletia indica) of Wheat" @default.
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