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- W2185647941 abstract "Now a day it is very important to remove industrial pollutants with minimum cost. The present work focused on the assessment of extracellular laccases produced by different fugal strains and its potential for Direct Green (DG) bio-removal from textile effluent water, which can be used as a resource of non-traditional water. Ten fungal strains from polluted sites were screened to test their capability of DG bioremoval to clean up waste water containing-dye. Fungal dye removal capacity was assessed by measuring the residual of DG dye in supernatant using spectrophotometer, over production of fungal biomass and reduction of COD values after 28h of incubation. Results showed that the fungal isolates could remove the simulated effluent containing-DG dye and they reduced the COD values indicating on bio-removal of dye from the growth media. Strain FRD 11 was the most effective strain which removed about 85% of (300 mgl -1 ) dye in 28h. Molecular characterization of fungal isolates was done by using the restriction fragment length polymorphisms (RFLP) and sequencing of ITS rRNA region as a reproducible molecular method for fungal species identification and differentiation. RFLP of 16S rRNA with Cfo I proved the suitability of this technique for strain typing, species identification and could divide them into three genetic clusters. Cluster 1 included strains (FRD1, 2, 3 and 5), cluster 2 (FRD 4, 7, 8, 10 and 11) and cluster 3 (FRD 9). Phylogenetic tree of ITS rRNA of five representative strains showed that strains FRD 7 and 11 were identified as Aspergillus tubigenesis , strain FRD 9 was identified as Aspergillus niger and strains FRD1 and 3 were belong to Aspergillus terreus . These results indicated that these fascinating new fungal strains can play significant role in bioremediation and detoxification of effluents polluted with DG dye due to their ability to biosorb or produce laccase enzyme-remediating dye." @default.
- W2185647941 created "2016-06-24" @default.
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- W2185647941 date "2015-01-01" @default.
- W2185647941 modified "2023-10-17" @default.
- W2185647941 title "Biodiversity Among Dominant Fungi Involved in Water Production from Non-Traditional Water Resources" @default.
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