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- W2185710711 abstract "SUMMARY I. Oxygen exchange and fluorescence transient studies, made with the intact cells of red alga Porphyridium cruentum, suggest that hydroxylamine (NH2OH) inactivates the oxygen evolving capacity; at high enough concentrations, it also feeds electrons to the reductant side of Photosystem II. This result is in confirmity with the studies made earlier with spinach chloroplasts and green alga Chlorella by other workers. Fluorescence transient data further indicate that the maximal rate of feeding of electrons by this reductant to Photosystem II is not as high as by water. 2. In Porphyridium, the early phase of the fluorescence transient is not markedly changed by NH~OH, but characteristic slow complex decay part of the fluorescence transient is completely abolished. However, the treated cells, like the normal cells, do need a dark exposure before subsequent illumination to fully restore the transient. NH2OH, at substrate concentrations, eliminates the recovery of the fluorescence rise curve (OI) in 3-(3',4'-dichlorophenyl)-I,I dimethyl urea (DCMU)-treated cells. Also NH20H together with DCMU abolishes all delayed light emission measured after a msec of illumination. These observations indicate that NH2OH inhibits the chemical back recombination reaction between Z ÷ (the primary oxidant produced by light reaction II) and Q- (the primary reductant produced by light reaction II). These results, obtained with Porphyridium were obtained independently of BENNOUN (1970) who reached similar conclusions from his studies with green alga Chlorella. 3. Furthermore, the feeding of electrons by NI-[2OH appears to be very close to the reaction center of Photosystem II, as the photooxidation of NH2OH quenches the 17696 band which has been suggested to be linked with the reaction center II." @default.
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- W2185710711 date "1971-01-01" @default.
- W2185710711 modified "2023-09-27" @default.
- W2185710711 title "ACTION OF HYDROXYLAMINE IN THE RED ALGA PORPHYRIDIUM" @default.
- W2185710711 hasPublicationYear "1971" @default.
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