Matches in SemOpenAlex for { <https://semopenalex.org/work/W2185727325> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2185727325 endingPage "71" @default.
- W2185727325 startingPage "266" @default.
- W2185727325 abstract "In Southeast Asia Trypanosoma evansi infection is a disease of economic importance since it affects the health of buffalo, cattle and swine. The acute stage symptoms include abortion, central nervous system disorder and even death, and in the chronic condition working capacity and productivity of the animals are affected. A polymerase chain reaction (PCR)-based detection technique has been developed with a sensitivity of 0.5 pg of parasite DNA or one single parasite in 10 microliters of blood samples which were allowed to clot and then boiled before DNA amplification. This permitted storage of blood collection at ambient temperature for at least one month. Phosphate-saline-glucose solution, normally used as trypanosome maintenance buffer, inhibited PCR. Although DNA primers used were derived from T. evansi specific sequence, amplification of the genome of T. brucei and T. equiperdum generated the same 227 bp fragment. This method should now make it possible to detect infections in livestock in the very early stages where microscope examination is equivocal and to monitor groups of animals after trypanocidal treatment." @default.
- W2185727325 created "2016-06-24" @default.
- W2185727325 creator A5035083670 @default.
- W2185727325 creator A5036381505 @default.
- W2185727325 creator A5051790930 @default.
- W2185727325 date "1994-06-01" @default.
- W2185727325 modified "2023-09-23" @default.
- W2185727325 title "A simplified and highly sensitive detection of Trypanosoma evansi by DNA amplification." @default.
- W2185727325 cites W1970144610 @default.
- W2185727325 cites W1992760440 @default.
- W2185727325 cites W2002011086 @default.
- W2185727325 cites W2028165445 @default.
- W2185727325 cites W2073867453 @default.
- W2185727325 cites W2078886897 @default.
- W2185727325 cites W2084221521 @default.
- W2185727325 cites W2085851942 @default.
- W2185727325 cites W2086349603 @default.
- W2185727325 cites W2132329283 @default.
- W2185727325 cites W2144634347 @default.
- W2185727325 cites W251956887 @default.
- W2185727325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7855639" @default.
- W2185727325 hasPublicationYear "1994" @default.
- W2185727325 type Work @default.
- W2185727325 sameAs 2185727325 @default.
- W2185727325 citedByCount "22" @default.
- W2185727325 countsByYear W21857273252012 @default.
- W2185727325 countsByYear W21857273252013 @default.
- W2185727325 countsByYear W21857273252014 @default.
- W2185727325 countsByYear W21857273252016 @default.
- W2185727325 countsByYear W21857273252021 @default.
- W2185727325 countsByYear W21857273252022 @default.
- W2185727325 crossrefType "journal-article" @default.
- W2185727325 hasAuthorship W2185727325A5035083670 @default.
- W2185727325 hasAuthorship W2185727325A5036381505 @default.
- W2185727325 hasAuthorship W2185727325A5051790930 @default.
- W2185727325 hasConcept C104317684 @default.
- W2185727325 hasConcept C136764020 @default.
- W2185727325 hasConcept C153911025 @default.
- W2185727325 hasConcept C159047783 @default.
- W2185727325 hasConcept C24586158 @default.
- W2185727325 hasConcept C2778143080 @default.
- W2185727325 hasConcept C2780939464 @default.
- W2185727325 hasConcept C41008148 @default.
- W2185727325 hasConcept C49105822 @default.
- W2185727325 hasConcept C54355233 @default.
- W2185727325 hasConcept C552990157 @default.
- W2185727325 hasConcept C71928629 @default.
- W2185727325 hasConcept C86803240 @default.
- W2185727325 hasConceptScore W2185727325C104317684 @default.
- W2185727325 hasConceptScore W2185727325C136764020 @default.
- W2185727325 hasConceptScore W2185727325C153911025 @default.
- W2185727325 hasConceptScore W2185727325C159047783 @default.
- W2185727325 hasConceptScore W2185727325C24586158 @default.
- W2185727325 hasConceptScore W2185727325C2778143080 @default.
- W2185727325 hasConceptScore W2185727325C2780939464 @default.
- W2185727325 hasConceptScore W2185727325C41008148 @default.
- W2185727325 hasConceptScore W2185727325C49105822 @default.
- W2185727325 hasConceptScore W2185727325C54355233 @default.
- W2185727325 hasConceptScore W2185727325C552990157 @default.
- W2185727325 hasConceptScore W2185727325C71928629 @default.
- W2185727325 hasConceptScore W2185727325C86803240 @default.
- W2185727325 hasIssue "2" @default.
- W2185727325 hasLocation W21857273251 @default.
- W2185727325 hasOpenAccess W2185727325 @default.
- W2185727325 hasPrimaryLocation W21857273251 @default.
- W2185727325 hasRelatedWork W2016973606 @default.
- W2185727325 hasRelatedWork W2079183722 @default.
- W2185727325 hasRelatedWork W2103928730 @default.
- W2185727325 hasRelatedWork W2185727325 @default.
- W2185727325 hasRelatedWork W2317009758 @default.
- W2185727325 hasRelatedWork W2510431343 @default.
- W2185727325 hasRelatedWork W2616450109 @default.
- W2185727325 hasRelatedWork W305078324 @default.
- W2185727325 hasRelatedWork W94071994 @default.
- W2185727325 hasRelatedWork W2247963965 @default.
- W2185727325 hasVolume "25" @default.
- W2185727325 isParatext "false" @default.
- W2185727325 isRetracted "false" @default.
- W2185727325 magId "2185727325" @default.
- W2185727325 workType "article" @default.