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• W2185783960 abstract "A B S T R A C T http://www.ijcmas.com This study aimed to determine the frequency of occult HBV infection among patients with haematological disorders who received frequent blood transfusion. It included 60 HBsAg-negative patients with haematological disorders at the hematological department of Medical Research Institute Hospital who received frequent blood transfusion. Sera collected from all patients were tested for HBsAg, anti-HBc, anti-HCV by enzyme-linked immunosorbant assay and detection of HBV-DNA by PCR. The anti-HBc was detected in 43.3% of the patients; HBV- DNA was detected in 53.3% by SYBR green real time PCR in two different region of the genome with a high detection in the S region alone (28.3%) compared to the core region (13.3%). Conventional nested PCR amplifying the pol gene was positive in only 6 (10%) of the patients. With the use of HBsAg as the sole detection marker for HBV, there is a danger of HBV transmission through blood transfusion. Hence, more sensitive screening tests should be added to the routine blood donor screening test to eliminate most of the risk of unsafe blood donation." @default.
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• W2185783960 date "2015-01-01" @default.
• W2185783960 modified "2023-09-27" @default.
• W2185783960 title "Occult Hepatitis B virus infection in patients with blood diseases" @default.
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