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- W2185974700 abstract "The aim of this study was to establish an adventitious roots culture system for sterile plantlet segments of <em>P. tunicoides</em> and improved the accumulation of total saponins in cultured roots. <em>Psammosilene tunicoides</em> is a native Chinese plant with high commercial value as medicinal herb. Combination of NAA and IBA significantly affected the adventitious roots formation on agar-solided B<sub>5</sub> media and a maximal induction rate of 83% was obtained at 24±2°C with a photoperiod of 12 h. With a shaking of 110 rpm in darkness, transferring the detached adventitious roots to the growth regulator free 1/2 B<sub>5</sub> liquid media notably increased the biomass production compared to that on solid media over a 30-day-culture period. Further analyses showed that more saponins could be accumulated in the liquid culture than in the solid culture and the addition of exogenous oxalic acid to the liquid media could enhance the accumulation of total saponins in adventitious roots. These results suggested that adventitious roots culture will be an efficient alternative to the field cultivation of intact plants for the production of useful natural compounds from <em>P. tunicoides</em>." @default.
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- W2185974700 date "2013-02-15" @default.
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- W2185974700 title "Liquid Culture of Adventitious Roots is a Potential Alternative to Field Cultivation for <em>Psammosilene tunicoides</em>, a Rare and Endangered Endemic Medicinal Plant" @default.
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- W2185974700 doi "https://doi.org/10.19026/ajfst.5.3231" @default.
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