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• W2186008309 abstract "In contrast to results obtained in the rat kidney, stud- ies of H4ATPa5elocalization in the rabbit kidney have failed to demonstrate basolateral plasma mem- brane HATPase immunoreactivity In intercalated cells in the cortical collecting duct (CCD). Previous studies have relied on light microscopic immunoflu- orescence techniques, which have limited resolu- tion. Therefore, the immunogold procedure was used to localize HATPase in rabbit collecting ducts at the uitrastructural level. Rabbit kidneys were preserved In vivo with perlodate-lysine-paraformaldehyde or glutaraldehyde solutions, and samples of cortex were embedded in Lowicryl K4M. Thin sections were labeled for H'ATPase by the immunogoid procedure wIth a rabbit polyclonal antibody against the 70-kd subunit of bovine brain H'ATPase. Three patterns of localization of WATPase were observed. The majority of intercalated cells in the CCD exhibited label over cytoplasmic vesicles only. In these cells, no label was associated with either the apical or basolateral plasma membranes. In a second group of cells, label for WATPase was observed along the basolateral plasma membrane and over cytoplasmic vesicles throughout the cell. Rarely, intercalated cells with H4ATPase label along the apical plasma membrane and over the apical cytoplasmic vesicies were ob- served in the CCD. In the initial collecting tubule and" @default.
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• W2186008309 date "1994-01-01" @default.
• W2186008309 modified "2023-09-27" @default.
• W2186008309 title "Ultrastructural Localization of HATPase in Rabbit Cortical Collecting Duct1'2" @default.
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