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- W2186013037 abstract "(Hup+) Bradyrhizobium japonicum. Hydrogen oxidation byHup-hrphenotype USDA 61incowpea symbioses was significant becausehydrogen evolution fromnitrogen-fixing nodules was notdetected. Anexamination foruptakehydrogenase activity in soybean andcowpea with123strains diverse inorigin andserology identified 16Hup+and28Hup-hr phenotype strains; theremainder appeared tobeHup-.TheHup-hrphenotype was associated withserogroups 31,76,and94,while strains belonging toserogroups6,31,110,122,123,and38/115 were Hup+.Hup+strains ofthe123serogrouptyped positive withUSDA129-specific antiserum. Thepresenceoftheuptake hydrogenase protein incowpea bacteroids ofHup+strains was demonstrated withimmunoblot analyses byusing antibodies against the65-kDasubunit ofuptakehydrogenase purified fromstrain SR470.However,thehydrogenase protein ofHup-hr strains was notdetected. Results ofSouthern hybridization analyses withpHUlshowedthe region ofDNAwithhydrogenase genesamong Hup+strains tobesimilar. Hybridization was also obtained with Hup-hrstrains byusing a variety ofcloned DNA as probesincluding hydrogenase structural genes.Both hydrogenase structural genesalsohybridized withtheDNA offourHup-strains." @default.
- W2186013037 created "2016-06-24" @default.
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- W2186013037 date "1990-01-01" @default.
- W2186013037 modified "2023-09-26" @default.
- W2186013037 title "Evidence fora ThirdUptakeHydrogenase Phenotype among theSoybean Bradyrhizobia" @default.
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