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• W2186017265 abstract "Ribonucleic acid (RNA) is the most abundant form of organic phosphorus (P) in plant and microbial biomass and is therefore expected to be present in materials such as soils, sediments, composts, and manures. Phosphorus-31 nuclear magnetic resonance (31P NMR) spectroscopy is increasingly used to characterize organic P in these materials, usually following extraction into a mixture of sodium hydroxide and ethylenediaminetetraacetic acid (NaOH-EDTA). Under these alkaline conditions, RNA is hydrolysed, providing a distinctive pattern or “fingerprint” in the 31P NMR spectrum. Complete assignment of the eight ribonucleotides produced was achieved using a spiking approach. The near coincidence in chemical shift of β-glycerophosphate and two of the ribonucleotide peaks complicates quantification of RNA concentrations when phospholipids are also present, but an approach based on quantifying signal in the most well-separated ribonucleotide peaks is suggested." @default.
• W2186017265 created "2016-06-24" @default.
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• W2186017265 date "2015-10-12" @default.
• W2186017265 modified "2023-09-27" @default.
• W2186017265 title "Identification of RNA Hydrolysis Products in NaOH-EDTA Extracts using31P NMR Spectroscopy" @default.
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• W2186017265 doi "https://doi.org/10.1080/00103624.2015.1093640" @default.
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