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- W2186188982 abstract "There is no cross expression of genes between Bacteroides sp. and Escherichia coli. In this study, the promoter structure and function of a strong housekeeping B. thetaiotaomicron 16S rRNA promoter region was examined and compared to one from E. coli in vivo using a newly developed Bacteroides lux reporter system. Analysis of the B. thetaiotaomicron sequences upstream of the 16S rRNA gene has revealed the same overall structure known for E. coli 16S rRNA promoters in that there are two putative promoters separated by approximately 150 bp. However, the B. thetaiotaomicron 16S rRNA promoter contains the proposed Bacteroides –7 and –33 consensus sequences instead of the well known E. coli –10 and –35 consensus sequences. The biological activity of various configurations of the B. thetaiotaomicron 16S rRNA promoter was analyzed. In addition, by pairing the B. thetaiotaomicron 16S rRNA promoter with an E. coli ribosomal binding site, and vice-versa, we further confirmed that the difference in gene expression between the two species lies at the level of transcription. However, in Bacteroides, barriers to both transcription and translation initiation appear to limit expression of foreign genes." @default.
- W2186188982 created "2016-06-24" @default.
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- W2186188982 date "2008-07-30" @default.
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- W2186188982 title "Studies of Three Human Intestinal Opportunistic Pathogens" @default.
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