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• W2186193159 abstract "6 RESUMO - Introducao: Treponema pallidun e o agente etiologico da sifilis uma doenca sexualmente transmissivel. No imunodiag- nostico dessa doenca utilizam-se dois diferentes tipos de testes sorologicos. Inicialmente, as amostras sao triadas qualitativamente e quantitativamente por um teste nao treponemico como o veneral disease research laboratory (VDRL) e, em seguida, os soros reagen- tes sao testados para a deteccao de anticorpos especificos para o Treponema pallidum, como a reacao de imunofluorescencia indireta atraves do fluorescent treponemal antibody absorption assay (FTA-ABS). Objetivos e Metodologia: Avaliar atraves do VDRL quanti- tativo e qualitativo a sororeatividade de 40 pacientes com sifilis e comparar com a presenca do fenomeno pro-zona e resultados obti- dos pelo FTA-ABS. Resultados e Discussao: Os niveis de reatividade das amostras testadas pelo VDRL variaram de 1:2 a 1:256. O fe- nomeno pro-zona foi observado em 8/40 soros (20%) se correlacionando com altos titulos de reatividade na maioria dos casos. O FTA- ABS foi reagente em 39/40 amostras (97,5%) apresentando forte e moderada reatividade independente dos titulos obtidos pelo VDRL e a presenca ou ausencia do fenomeno pro-zona na maioria dos casos, demonstrando uma maior sensibilidade desse exame quando comparado com o VDRL. Conclusao: Nossos resultados mostraram a importância da realizacao do VDRL quantitativo e qualitativo na investigacao seguida pelo FTA-ABS na investigacao sorologica da sifilis. PALAVRAS-CHAVE - Sifilis, Treponema pallidum, Veneral Disease Research Laboratory, Fluorescent Treponemal Antibody Absorp- tion Assay. SUMMARY - Introduction: Treponema pallidum is the aetiological agent of syphilis, a sexually transmitted disease and the serology is essential for screening and diagnosis. In the immunediagnosis of this disease, two different sorts of serological tests are used. First the sera are screened in a quantitative and qualitative manner by a nontreponemal test such as the veneral disease research labora- tory test (VDRL), then the reactive sera are tested for specific treponemal antibodies using the fluorescent treponemal antidody ab- sorption assay (FTA-ABS). Objecitves and Methodology: To evaluate, using quantitative and qualitative VDRL, the sera reactivity of 40 patients bearing syphilis and compare these results to presence of prozone phenomenon and FTA-ABS. Results and discussion: The levels of sera reactivity in the VDRL test ranged from 1:2 to 1:256. The prozone phenomenon was observed in 8/40 amples (20%) and was correlated with high levels of VDRL in most of the cases. The FTA-ABS test was reactive in 39/40 cases (97,5%) with strong and moderate fluorescence intensity in most of the samples and it was independent of the VDRL reactivity levels and the presence or ab- sence of prozone phenomenon in mos of the cases, demonstrating a higher sensibility of this method when compared to the VDRL test. Conclusion: Our results showed the importance of qualitative and quantitative VDRL assay in the serological investigation of syphilis." @default.
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• W2186193159 date "2006-01-01" @default.
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• W2186193159 title "Caracterização e correlação do fenômeno pró-zona com títulos de sororeatividade do VDRL e reação de imuno- fluorescência indireta em soros de pacientes com sífilis Characterization and correlation of prozone phenomenon whit seroreactivity and indirect immunofluorescence in sero from patients with syphilis" @default.
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