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- W2186193775 abstract "The detection of viral genome through the use of polymerase chain reaction (PCR) has made possible a new step in viral diagnosis. The main advantages of PCR are its extreme sensitivity and the possibility to develop rapid assays using non radioactive probes. The amplification of hepatitis B virus (HBV) DNA sequences in sera for the diagnosis of HBV is an important application of PCR with regard to HBV. A set of primer B2833S 5` GGGTCACCATATTCTTGG 3` and B170AS 5` GT CCTAGGAATCCTGATG was tested for the amplification of HBV genome from the plasma derived HBV patients and a cloned HBV. Annealing temperature and units of Taq polymerase were optimized. Annealing temperature 55°C was found best for B2833S and B170AS. It was established that one unit of Taq polymerase amplify optimally in a reaction mixture of 25 μl. This procedure will be more reliable evidence of the presence of Hepatitis B virus." @default.
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- W2186193775 date "2005-01-01" @default.
- W2186193775 modified "2023-10-03" @default.
- W2186193775 title "PCR Based Diagnosis of Hepatitis B Virus" @default.
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