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- W2186193800 abstract "The antimicrobial and toxicity against Artemia sali na Leach of extracts from different parts (stems, l eaves and seeds) of Ocimum gratissimum collected at two veget ative stages were analyzed. In addition to essentia l oils that contain volatile compounds and which antimicrobial properties we studied in a previous work [12], the plant contains less or non-volatile compounds that could explain in part, its properties. The crude ethanol extract of different parts (leaves, stems and seeds in pre- an d full flowering) showed yields between 1.97% and 4Leaves had given more extract than seeds and the lower yie ld was obtained with the stems. Antimicrobial test s and toxicity against A. salina Leach helped to identify the crud e extract of leaves and stems in full flowering as the most active respectively against C. albicans ATCC, and E. coli ATCC and S. aureus ATCC with a good selectivity. Phytochemical screening of different samples reveal ed the presence of polyphenolic compounds (gallic a nd catechic tannins, flavonoids, anthocyanes and leucoanthocyan es), quinone derivatives, triterpenoids, steroids, mucilage, coumarins, reducing compounds and essential oils. The liquid-liquid fractionation of the active crude extracts with different solvents of increasing polarity showed et hyl acetate fraction as the most active. This fract ion needs to be further investigated by isolation and identificatio n of pure bioactive compounds by bio-guided fractio nation. This is the first report of interaction between plant parts , vegetative stages, antimicrobial properties and tof the nonvolatile fractions of O. gratissimum Linn from Benin." @default.
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- W2186193800 date "2013-01-01" @default.
- W2186193800 modified "2023-10-12" @default.
- W2186193800 title "Phytochemical screening, antimicrobial activities a nd toxicity against Artemia salina Leach of extracts and fractions of Ocimum gratissimum Linn from Benin" @default.
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