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- W2186246162 abstract "22 23 Rapid and cost-effective MALDI-TOF MS-based systems will replace conventional 24 phenotypic methods for routine identification of bacteria. We report here the first evaluation 25 of the new MALDI-TOF MS-based VITEK MS system in a large clinical microbiology 26 laboratory. This system used an original spectra classifier algorithm and a specific database 27 designed for the identification of clinically relevant species. 28 We have tested 767 routine clinical isolates representative of 50 genera and 124 29 species. VITEK MS-based identifications were performed by means of a single deposit on 30 MALDI disposable target, without any prior extraction step, and compared with reference 31 identifications, mainly obtained with the VITEK2 phenotypic system; if discordant, molecular 32 techniques provided reference identifications. 33 The VITEK MS system provided 96.2% of correct identifications: to the species level 34 (86.7%), to the genus level (8.2%), or within a range of species belonging to different genera 35 (1.3%). Conversely, 1.3% of isolates were misidentified and 2.5% unidentified, partly 36 because the species was not included in the database; a second deposit provided a successful 37 identification for 0.8% of isolates unidentified with the first deposit. 38 The VITEK MS system is a simple, convenient and accurate method for routine bacterial 39 identification with a single deposit, considering the high bacterial diversity studied as 40 evidence by the low prevalence of species without correct identification. In addition to a 41 second deposit in uncommon cases, expanding the spectral database is expected to further 42 enhance performances. 43 44" @default.
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- W2186246162 date "2012-01-01" @default.
- W2186246162 modified "2023-09-25" @default.
- W2186246162 title "Performances of the MALDI-TOF Mass Spectrometry system" @default.
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