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- W2186265852 abstract "Synopsis--An outline of the principles underlying fluorescent antibody techniques is given. In dermatology, these methods have been used as histological markers in studies of the antigenicity of connective tissue and in the investigation of the possible role of auto-immune factors in diseases involving the skin. They have also been used to demonstrate the serological abnormalities which are a characteristic feature of many auto-immune diseases. In recent years fluorescent antibody techniques have been used in many fields of medicine, both as research tools and as diagnostic aids. Immunological reactions are useful because of their specificity and sensitivity. Coons et al (1,2), who introduced the fluorescent antibody technique, found that antibody globulin can be chemically combined with fluorescent dyes such as fluorescein isocyanate without losing its biological or immunological properties. Such conjugates could be used as histological markers to indicate the sites of reactions between antibody and antigen in tissue sections or cell smears. The choice of a fluorescent label allows the site of such reactions to be demonstrated when sections are examined in UV light. Fluorescent dyes are used because they can be detected in a lower concentration than ordinary dyes. The Coons' fluorescent antibody technique has been used in the study of antigens in bacteria, viruses, protozoa, fungi and in animal and human tissues. It is also suitable for investigation of the presence of antibody/n tissue and in serum." @default.
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- W2186265852 date "1966-01-01" @default.
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- W2186265852 title "Fluorescent antibody in dermatology techniques" @default.
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