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- W2186307954 abstract "We are developing field-portable instruments and disposable fluid cartridges that enable the rapid and quantitative analysis of target bacteria or viruses in complex mixtures. Up to many milliliters of raw sample are processed automatically in a flow-through manner, and processing functions are sequentially effected. These include specimen dilution, coarse filtration, target microbe extraction/separation, microbe lysis with or without chemicals and/or heat, neutralization, DNA/RNA purification and concentration, PCR reagent mixing, and PCR or RT-PCR. As part of the development of this system, we have designed and tested a silicon-based fluidic chip that extracts DNA from 500 uL of fluid and dilutes into 25-50 uL at greater than 50% efficiency and resulting in a 10- to 20-fold increase in concentration. This operation can be completed in several minutes. We have also designed, built, and tested complex fluidic cartridges with large specimen volume capacity, integral waste regions, filters, heaters, integrated DNA chips, fluid divertors, and PCR reaction tube filling. Rapid PCR modules, capable of 7-10 °C/sec and 2-5°C/sec heating and cooling, respectively for 100 uL reaction volumes and 2 or 4-color real-time fluorescence detection suitable for Taqman®, Molecular Beacons, and other homogeneous chemistry systems, have been built and demonstrated." @default.
- W2186307954 created "2016-06-24" @default.
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- W2186307954 date "1999-01-01" @default.
- W2186307954 modified "2023-09-26" @default.
- W2186307954 title "Application of advanced microfluidics and rapid PCR to analysis of microbial targets" @default.
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