Matches in SemOpenAlex for { <https://semopenalex.org/work/W2186535270> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2186535270 abstract "The femtosecond laser was used for simultaneous single-beam three-dimensional optical trapping of: and excitation of second-harmonic as well as two-photon fluorescence in biotin and biotin ester microcrystals. The single-beam gradient-force optical trap is an efficient method for manipulating and positioning microparticles. In this case the laser acts simultaneously as the trapping beam and the fundamental beam for the second-harmonic generation and two-photon fluorescence. The particles are trapped just below the focus as a result of the gradient forces generated by radiation pressure and scattering forces (l). Biotin or Vitamin H and its' derivations are commonly used in a variety of biochemical applications. Recently the SHG (Z) and two-photon (2p) fluorescence (3,4) detection of bio-objects is developed and wide- used method of investigation of biological important substances and structures. The combination of trapping and excitation of SHG and 2p-autofluorescence of biotin ester microcrystals allow us to investigate the structure and optic properties of it. Additionally, the particles with submicron size are useful as a non-intrusive microscopic probe for high-resolution studies(5). In this study Zp-fluorescence and SHG from microcrystals of biotin and its derivations were observed in femtosecond conditions of excitation in wide spectral range at simultaneous optical trapping. To our knowledge, this is th: first observation of SHG in microcrystals of biotin and biotin ester as well as is the first realization of single-beam three-dimensional optical trapping and SHG and 2p-fluorescence in femtosecond conditions of excitation. The water suspensions of microcrystals of biotin and biotin ester (SIGMA) were used. A femtosecond Tksapphire laser (Mira-900 Coherent) pumped by a solid-state laser (Verdi, Coherent) was used to trap the microcrystals and to excite SHG and 2p-fluorescence. The laser wavelength was tuned over in near IR range 755-930 nm. The average incident laser beam power in focal spot varied in range 3-35 mW. For microscopic imaging, a modified laser confocal microscope (FV 300, Olympus) employing photomultiplier tubes as detectors was used. The reflective and SHG microscopic images of the same microcrystals in transmission direction were also obtained. The intensity and duration of SH and Zp-fluorescent signals emitting were comparatively analyzing for biotin' and biotin ester and for different conditions of experiment (the microcrystal size, trapping; durations, intensity, wavelength of excitation). The Zp-fluorescence and SHG of biotins also were compared with Zp- fluorescence of other biologcally important substances -vitamins, proteins, etc. The biotin ester trapped microcrystals generate intensive and stable second harmonic for the whole laser tuning range in 377.5-465 nm as well as they exhibit two-photon fluorescence in spectral range 510-530 nm. Such microcrystals can be manipulated and positioned by optical trapping and used as a microprobe for high- resolution investigation. Biotin microcrystals also exhibit 2p-fluorescense and intensive SHG, but not so stable" @default.
- W2186535270 created "2016-06-24" @default.
- W2186535270 creator A5006062349 @default.
- W2186535270 creator A5016747836 @default.
- W2186535270 creator A5018517116 @default.
- W2186535270 creator A5021732322 @default.
- W2186535270 date "2003-01-01" @default.
- W2186535270 modified "2023-09-27" @default.
- W2186535270 title "NONLINEAR OPTICAL SPECTROSCOPY OF BIOTIN AND BIOTIN HARMONIC GENERATION" @default.
- W2186535270 cites W1979968811 @default.
- W2186535270 cites W2135446345 @default.
- W2186535270 hasPublicationYear "2003" @default.
- W2186535270 type Work @default.
- W2186535270 sameAs 2186535270 @default.
- W2186535270 citedByCount "0" @default.
- W2186535270 crossrefType "journal-article" @default.
- W2186535270 hasAuthorship W2186535270A5006062349 @default.
- W2186535270 hasAuthorship W2186535270A5016747836 @default.
- W2186535270 hasAuthorship W2186535270A5018517116 @default.
- W2186535270 hasAuthorship W2186535270A5021732322 @default.
- W2186535270 hasConcept C120665830 @default.
- W2186535270 hasConcept C121332964 @default.
- W2186535270 hasConcept C158711907 @default.
- W2186535270 hasConcept C167735695 @default.
- W2186535270 hasConcept C179687394 @default.
- W2186535270 hasConcept C185592680 @default.
- W2186535270 hasConcept C192562407 @default.
- W2186535270 hasConcept C2778204606 @default.
- W2186535270 hasConcept C30713254 @default.
- W2186535270 hasConcept C47785574 @default.
- W2186535270 hasConcept C520434653 @default.
- W2186535270 hasConcept C55493867 @default.
- W2186535270 hasConcept C62520636 @default.
- W2186535270 hasConcept C83581075 @default.
- W2186535270 hasConcept C91881484 @default.
- W2186535270 hasConceptScore W2186535270C120665830 @default.
- W2186535270 hasConceptScore W2186535270C121332964 @default.
- W2186535270 hasConceptScore W2186535270C158711907 @default.
- W2186535270 hasConceptScore W2186535270C167735695 @default.
- W2186535270 hasConceptScore W2186535270C179687394 @default.
- W2186535270 hasConceptScore W2186535270C185592680 @default.
- W2186535270 hasConceptScore W2186535270C192562407 @default.
- W2186535270 hasConceptScore W2186535270C2778204606 @default.
- W2186535270 hasConceptScore W2186535270C30713254 @default.
- W2186535270 hasConceptScore W2186535270C47785574 @default.
- W2186535270 hasConceptScore W2186535270C520434653 @default.
- W2186535270 hasConceptScore W2186535270C55493867 @default.
- W2186535270 hasConceptScore W2186535270C62520636 @default.
- W2186535270 hasConceptScore W2186535270C83581075 @default.
- W2186535270 hasConceptScore W2186535270C91881484 @default.
- W2186535270 hasLocation W21865352701 @default.
- W2186535270 hasOpenAccess W2186535270 @default.
- W2186535270 hasPrimaryLocation W21865352701 @default.
- W2186535270 hasRelatedWork W2008679154 @default.
- W2186535270 hasRelatedWork W2014477744 @default.
- W2186535270 hasRelatedWork W2027747973 @default.
- W2186535270 hasRelatedWork W2028451105 @default.
- W2186535270 hasRelatedWork W2046865503 @default.
- W2186535270 hasRelatedWork W2061470858 @default.
- W2186535270 hasRelatedWork W2066142635 @default.
- W2186535270 hasRelatedWork W2077313912 @default.
- W2186535270 hasRelatedWork W2086333091 @default.
- W2186535270 hasRelatedWork W2111027336 @default.
- W2186535270 hasRelatedWork W2130010119 @default.
- W2186535270 hasRelatedWork W2131522388 @default.
- W2186535270 hasRelatedWork W2151366429 @default.
- W2186535270 hasRelatedWork W2158379815 @default.
- W2186535270 hasRelatedWork W2199534296 @default.
- W2186535270 hasRelatedWork W2978022955 @default.
- W2186535270 hasRelatedWork W3038821139 @default.
- W2186535270 hasRelatedWork W1674452757 @default.
- W2186535270 hasRelatedWork W2470246216 @default.
- W2186535270 hasRelatedWork W2523561154 @default.
- W2186535270 isParatext "false" @default.
- W2186535270 isRetracted "false" @default.
- W2186535270 magId "2186535270" @default.
- W2186535270 workType "article" @default.