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- W2186545848 abstract "METHODS: Samples from patients attending Ontario medical clinics were Pap,tested and within 1 week the residual L,Pap fluid from patients with abnormal cytology were extracted in QlAamp MinElute columns. Amplification and detection of HR HPV and beta,globin DNA were per- formed according to the package inserts. For the reproducibility protocol the two laboratories extracted and tested a panel of samples. Analysis was performed using SPSS software. RESULTS: A total of 325 women have been enrolled (49% >30 yr). Sixty,five percent (212) contained HR types. The following genotype prevalences were found: 16 (24%). 18 (8%), 51 (11%), 52 (10%), 39 (9%), 56 (8%),31 or 58 (6%), 33 (4%), 35, 45 or 68 (3%), 59 (2%). Of the 212 samples containing an HR genotype, 44 had 2, 17 had 3, 7 had 4, and 1 had 5 different types. HPV DNA was detected in 100% (15/15) samples with HSIL, 83.0% with ASCUS (78/94) and 81.7% with LSIL (107/131). In patients 30. The tests agreed 79.7% of the time with the AMP test finding more positives due to a higher analytical sensitivity. The 2 laboratories agreed on positives and negatives 76.2% of the time. CONCLUSIONS: This ongoing study showed that although infections with types 31,39, 51, 52, 56 or 58 were prevalent in 52% of the positive samples, they were often accompanied by types 16 and 18, which were more often associated with cytological diagnoses of ASCUS or HSIL. Results of AMP testing between laboratories may be due to differences in extraction and/or performance of the assay." @default.
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- W2186545848 date "2008-01-01" @default.
- W2186545848 modified "2023-10-11" @default.
- W2186545848 title "PREVALENCEOF HIGH RISK HPV GENOTYPES IN ONTARIO WOMEN DETERMINED BY AMPLlCOR@ HPV PCR AND LINEAR ARRAY OF SUREPATH@ PAP SAMPLES" @default.
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