Matches in SemOpenAlex for { <https://semopenalex.org/work/W2186761805> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2186761805 abstract "Summary Background: cryopreservationdecreasesspermviabilitybyapproximately50%. Objective: the objective of this study was to determine the effect of the addition of seminal plasma proteins on post-thawing sperm viability in Sanmartinero and Zebu semen. Methods: semen samples from 10 bulls of each breed were used, and seminal plasma was subjected to two-dimensional electrophoresis to establish the relationship between the relative amount of each protein spot and sperm viability. Then, seminal plasma was subjected to exclusion chromatography to separate the fraction containing these proteins.This fraction was added in doses of 0.5, 1.0, 1.5 and 2.0 mg, to 1 x 10 6 . Sperm was thawed and incubated at 37 °C for 1 h to determine its effect on postthaw viability. Sperm were frozen using two media (citrate-fructose-yolk and Bioxcell ® ). Results: we found one protein spot (16.20 kDa, PI 5.5) in Sanmartinero seminal plasma that correlated (r = 0.64 p<0.001) with viability. This spot was found in 21-25 chromatography fractions. The percentage of post-thaw viable sperm increased 20% (p<0.05) at 1.0 and 1.5 mg of the fraction when sperm was frozen using citrate-fructose-yolk; it increased 25% (p<0.01) with 0.5 mg when it was frozen with Bioxcell ® media. Addition of 0.5 mg of the fraction to semen cryopreserved with Bioxcell ® resulted in a greater (p<0.05) percentage increase of viable sperm in Sanmartinero semen (23 ± 8.3%) compared with Zebu semen (6.0 ± 2.0%). Conclusions: these" @default.
- W2186761805 created "2016-06-24" @default.
- W2186761805 creator A5003096412 @default.
- W2186761805 creator A5015019340 @default.
- W2186761805 creator A5029854679 @default.
- W2186761805 creator A5036862982 @default.
- W2186761805 creator A5039104563 @default.
- W2186761805 creator A5065577002 @default.
- W2186761805 creator A5089409739 @default.
- W2186761805 date "2013-01-01" @default.
- W2186761805 modified "2023-09-27" @default.
- W2186761805 title "Increase in post-thaw viability by adding seminal plasma proteins to Sanmartinero and Zebu sperm ¤ Incremento en la viabilidad espermática post-descongelación por la adición de proteínas del plasma seminal a semen de toros Sanmartinero y Cebú Aumento da viabilidade espermática pós-descongelamento, com a adição de proteínas do plasma seminal de sêmen de touros das raças Sanmartinero e Zebu" @default.
- W2186761805 cites W1520985997 @default.
- W2186761805 cites W1579024497 @default.
- W2186761805 cites W1964048757 @default.
- W2186761805 cites W1983880719 @default.
- W2186761805 cites W2017709734 @default.
- W2186761805 cites W2049678119 @default.
- W2186761805 cites W2073371105 @default.
- W2186761805 cites W2103650850 @default.
- W2186761805 cites W2107071527 @default.
- W2186761805 cites W2109500270 @default.
- W2186761805 cites W2111527733 @default.
- W2186761805 cites W2114551444 @default.
- W2186761805 cites W2117163228 @default.
- W2186761805 cites W2134513642 @default.
- W2186761805 cites W2134900948 @default.
- W2186761805 cites W2145120347 @default.
- W2186761805 cites W2164578937 @default.
- W2186761805 cites W2164826945 @default.
- W2186761805 cites W2404098128 @default.
- W2186761805 cites W72410149 @default.
- W2186761805 cites W968590969 @default.
- W2186761805 cites W1977308929 @default.
- W2186761805 hasPublicationYear "2013" @default.
- W2186761805 type Work @default.
- W2186761805 sameAs 2186761805 @default.
- W2186761805 citedByCount "0" @default.
- W2186761805 crossrefType "journal-article" @default.
- W2186761805 hasAuthorship W2186761805A5003096412 @default.
- W2186761805 hasAuthorship W2186761805A5015019340 @default.
- W2186761805 hasAuthorship W2186761805A5029854679 @default.
- W2186761805 hasAuthorship W2186761805A5036862982 @default.
- W2186761805 hasAuthorship W2186761805A5039104563 @default.
- W2186761805 hasAuthorship W2186761805A5065577002 @default.
- W2186761805 hasAuthorship W2186761805A5089409739 @default.
- W2186761805 hasConcept C140793950 @default.
- W2186761805 hasConcept C16685009 @default.
- W2186761805 hasConcept C179933525 @default.
- W2186761805 hasConcept C185592680 @default.
- W2186761805 hasConcept C196843134 @default.
- W2186761805 hasConcept C2776247511 @default.
- W2186761805 hasConcept C2776482104 @default.
- W2186761805 hasConcept C2776970464 @default.
- W2186761805 hasConcept C2778093475 @default.
- W2186761805 hasConcept C2781087480 @default.
- W2186761805 hasConcept C2781321622 @default.
- W2186761805 hasConcept C31903555 @default.
- W2186761805 hasConcept C55493867 @default.
- W2186761805 hasConcept C71924100 @default.
- W2186761805 hasConcept C86803240 @default.
- W2186761805 hasConcept C95444343 @default.
- W2186761805 hasConceptScore W2186761805C140793950 @default.
- W2186761805 hasConceptScore W2186761805C16685009 @default.
- W2186761805 hasConceptScore W2186761805C179933525 @default.
- W2186761805 hasConceptScore W2186761805C185592680 @default.
- W2186761805 hasConceptScore W2186761805C196843134 @default.
- W2186761805 hasConceptScore W2186761805C2776247511 @default.
- W2186761805 hasConceptScore W2186761805C2776482104 @default.
- W2186761805 hasConceptScore W2186761805C2776970464 @default.
- W2186761805 hasConceptScore W2186761805C2778093475 @default.
- W2186761805 hasConceptScore W2186761805C2781087480 @default.
- W2186761805 hasConceptScore W2186761805C2781321622 @default.
- W2186761805 hasConceptScore W2186761805C31903555 @default.
- W2186761805 hasConceptScore W2186761805C55493867 @default.
- W2186761805 hasConceptScore W2186761805C71924100 @default.
- W2186761805 hasConceptScore W2186761805C86803240 @default.
- W2186761805 hasConceptScore W2186761805C95444343 @default.
- W2186761805 hasLocation W21867618051 @default.
- W2186761805 hasOpenAccess W2186761805 @default.
- W2186761805 hasPrimaryLocation W21867618051 @default.
- W2186761805 isParatext "false" @default.
- W2186761805 isRetracted "false" @default.
- W2186761805 magId "2186761805" @default.
- W2186761805 workType "article" @default.