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- W2186780327 abstract "A respiratory disease outbreak was reported in layer flocks in and around Sammundri area of Punjab. Postmortem of the affected birds were performed and morbid organs (trachea, lungs, air sacs, spleen, proventriculus and brain) were collected and processed for isolation and identification of etiological agent. The samples were inoculated in 9-days old embryonated hen eggs. The harvested amnio-allantoic fluid (AAF) agglutinated chicken RBCs and was subjected to inhibition of haemagglutination (HI) and Agar Gel Precipitation test (AGPT) with specific antisera of Newcastle Disease Virus (NDV), H9 and H7 serotype of Avian Influenza (AI) virus to identify the etiological agents. The haemagglitination (HA) activity of AAF collected from the samples of Sammundri and Gojra areas were inhibited with antisera of NDV while antiserum of AIV H9 inhibited the HA activity of AAF of Kamalia area. AGPT with known antisera confirmed the presence of NDV in the outbreaks of Sammundri, Gojra where as AIVH9 in the outbreaks of Kamalia area. Isolated NDV was pathotyped as velogenic on the basis of Mean Death Time (MDT), Intracerebral Pathogenicity Index (ICPI) and Intravenous Pathogenicity Index (IVPI). The serum samples collected were analyzed for antibodies to NDV, H7, H9 serotype of AI and Mycoplasma gallisepticum (MG). The sera of sammundri and Gojra areas were found positive to MG, NDV while that of Kamalia to MG and H9 serotype of AI. Bacteriological culture indicated E.coli and Salmonella infection in the flocks. The clinical signs, postmortem lesions, serological analysis and bacteriological examination indicated that a number of infectious agents like NDV, H9 serotype of AI along with, E.coli and Salmonella, were responsible for the recent respiratory outbreaks in layer flocks." @default.
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- W2186780327 date "2008-01-01" @default.
- W2186780327 modified "2023-09-27" @default.
- W2186780327 title "Study of Disease Outbreak in Layer Flocks in and Around Sammundri Area" @default.
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