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• W2186787952 endingPage "3764" @default.
• W2186787952 startingPage "3755" @default.
• W2186787952 abstract "In situ hybridization studies, promoter analyses and antisense RNA experiments have implicated transcription factor GATA-4 in the regulation of cardiomyocyte differentiation. In this study, we utilized Gata4-/- embryonic stem (ES) cells to determine whether this transcription factor is essential for cardiomyocyte lineage commitment. First, we assessed the ability of Gata4-/- ES cells form cardiomyocytes during in vitro differentiation of embryoid bodies. Contracting cardiomyocytes were seen in both wild-type and Gata4-/- embryoid bodies, although cardiomyocytes were observed more often in wild type than in mutant embryoid bodies. Electron microscopy of cardiomyocytes in the Gata4-/- embryoid bodies revealed the presence of sarcomeres and junctional complexes, while immunofluorescence confirmed the presence of cardiac myosin. To assess the capacity of Gata4-/- ES cells to differentiate into cardiomyocytes in vivo, we prepared and analyzed chimeric mice. Gata4-/- ES cells were injected into 8-cell-stage embryos derived from ROSA26 mice, a transgenic line that expresses beta-galactosidase in all cell types. Chimeric embryos were stained with X-gal to discriminate ES cell- and host-derived tissue. Gata4-/- ES cells contributed to endocardium, myocardium and epicardium. In situ hybridization showed that myocardium derived from Gata4-/- ES cells expressed several cardiac-specific transcripts, including cardiac alpha-myosin heavy chain, troponin C, myosin light chain-2v, Nkx-2.5/Csx, dHAND, eHAND and GATA-6. Taken together these results indicate that GATA-4 is not essential for terminal differentiation of cardiomyocytes and suggest that additional GATA-binding proteins known to be in cardiac tissue, such as GATA-5 or GATA-6, may compensate for a lack of GATA-4." @default.
• W2186787952 created "2016-06-24" @default.
• W2186787952 creator A5003470749 @default.
• W2186787952 creator A5017899142 @default.
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• W2186787952 date "1997-10-01" @default.
• W2186787952 modified "2023-10-14" @default.
• W2186787952 title "Cardiomyocyte differentiation by GATA-4-deficient embryonic stem cells" @default.
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• W2186787952 doi "https://doi.org/10.1242/dev.124.19.3755" @default.
• W2186787952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9367431" @default.
• W2186787952 hasPublicationYear "1997" @default.
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