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- W2186825710 abstract "The current study shows an improved mutant of S. cerevisiae selected for the production of Fructofuranosidase (Ffase) from different substrates of industrial waste products in a semi commercial scale digitally controlled bioreactor. Fermentation parameters, such as carbon sources and their concentrations (12-15%), nitrogen sources (0.2%), air flow rate (0.2 vvm), agitational intensity (300 rpm), additives (NaF) were optimized.This gamma ray-induced mutation has given a stable and viable mutant for hyper-production of invertase Ffase.The productivity is >2.0-fold improved over the parental strain on an industrial waste molasses-based medium for extracellular and intracellular Ffase production using semi commercial production conditions. This enhancement occurred due to increase in gene copy number and improvement in gene expression or both or improvement in transport system of the cells. The mechanism underlying this hyper- secretion is of paramount significance and needs further study. The mutant of S. cerevisiae has obvious advantage of hyper production of Ffase and may serve as a starting strain for further genetic improvement. It is concluded that this organism may be exploited for bulk production of invertase using molasses an inexpensive and abundant." @default.
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- W2186825710 date "2011-01-01" @default.
- W2186825710 modified "2023-09-27" @default.
- W2186825710 title "Enhanced Production of Invertase from Thermoltolerant Yeast Through Black Strap Molasses a Waste Prodcut of Sugar Industry" @default.
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