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- W2186852010 abstract "summary MSMEG_0307 is annotated as a transcriptional regulator belonging to the AraC protein family and is located adjacent to the arylamine N-acetyltransferase (nat) gene in Mycobacterium smegmatis, in a gene cluster, conserved in most environmental mycobacterial species. In order to elucidate the function of the AraC protein from the nat operon in M. smegmatis, two conserved palindromic DNA motifs were identified using bioinformatics and tested for protein binding using electrophoretic mobility shift assays with a recombinant form of the AraC protein. We identified the formation of a DNA:AraC protein complex with one of the motifs as well as the presence of this motif in 20 loci across the whole genome of M. smegmatis, supporting the existence of an AraC controlled regulon. To characterise the effects of AraC in the regulation of the nat operon genes, as well as to gain further insight into its function, we generated a DaraC mutant strain where the araC gene was replaced by a hygromycin resistance marker. The level of expression of the nat and MSMEG_0308 genes was down-regulated in the DaraC strain when compared to the wild type strain indicating an activator effect of the AraC protein on the expression of the nat operon genes. © 2014 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license" @default.
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- W2186852010 date "2014-01-01" @default.
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- W2186852010 title "NON-TUBERCULOUS MYCOBACTERIA: GENERAL Characterisation of a putative AraC transcriptional regulator from Mycobacterium smegmatis" @default.
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