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- W2186926407 abstract "The study examined the optimal pretreatment condition for enzymatic hydrolysis of Ulva pertusa kjellman in order to transfer to bioenergy. Acids were examined for the pretreatment and HCl was selected as a proper candidate. In the experiments of changing acid concentration and temperature, hydrolysis rate of 51.6% was obtained under the condition of 121 C for 15 min with 0.1 M HCl. High temperature condition had an large influence on the hydrolysis efficiency. The pretreated U. pertusa kjellman with HCl was followed by the enzymatic hydrolysis using Viscozyme or xylanase, which were efficient for the hydrolysis. The hydrolysis rates of two enzymes at condition of 0.1 Unit/ml were 52.5, 50.6%, respectively. When two enzymes were used under a mixture of 2 : 1, the hydrolysis rate of 58.8% was obtained. In consequence, it was tentatively determined that an optimal condition of enzymatic hydrolysis containing acid pretreatment of U. pertusa kjellman was the condition of 121 C for 15 min with 0.1 M HCl followed by enzymatic hydrolysis using the mixture of Viscozyme and Xylanase of 2 : 1. In addition, HPLC analysis of the hydrolite obtained from various pretreatment and hydrolysis conditions showed that sugars, such as rhamnose, xylose, glucose. fructose, were found in almost samples treated with enzymes, and that mannose and galactose were additively found in the sample treated with HCl. The results indicate that a strain available of C5 sugar as well as C6 should be employed for a bioenergy fermentation because the hydrolite is composed of the mixture of C5 and C6 sugars." @default.
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- W2186926407 date "2012-01-01" @default.
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- W2186926407 title "Characteristics of Enzymatic Hydrolysis of Ulva pertusa kjellman by Various Pretreatments" @default.
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