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- W2186949085 abstract "Aim-To developa highlysensitive and specific enzymelinkedimmunosorbent assay(ELISA)systemforanalysis ofp53 protein incancerlysates. Methods-Theanti-p53 monoclonal antibodiesD07,1801,BP53.12, and421,and anti-p53 polyclonal antiserum CM1 were assessed by immunohistochemistry and westernblotanalysis toidentify those mostsuitable fordetermining p53status ofcancercelis. Antibodies withdesired characteristics wereusedtodevelopa non-competitive sandwichtypeELISA systemforanalysis ofp53expression in cancercytosols. UsingtheELISA,p53 protein concentrations weremeasuredin a smallseries ofbreastcancers, andthe quantitative valuescomparedwithp53 immunohistochemical" @default.
- W2186949085 created "2016-06-24" @default.
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- W2186949085 date "1997-01-01" @default.
- W2186949085 modified "2023-09-27" @default.
- W2186949085 title "A novel quantitative immunoassay systemforp53 using antibodies selected foroptimumdesignation" @default.
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- W2186949085 hasPublicationYear "1997" @default.
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