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- W2187029791 abstract "Inverted repeat gene structures designed for silencing functional genes have been widely used both in academic and applied research. The correct orientations of such struc- tures are usually validated with restriction analysis and/or sequencing. We speculated that the inverted repeat nature of such constructs can be shown by a simple PCR reaction with a single forward primer. To test this hypothesis five different constructs were established from grapevine sequences in a hairpin-intron style silencing system. We were able to amplify the appropriate products in each case. Thus a forward-primed PCR alone may be sufficient to prove the inverted repeat nature of the desired constructs." @default.
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- W2187029791 date "2014-01-01" @default.
- W2187029791 modified "2023-09-24" @default.
- W2187029791 title "Detection of self-complementary inverted repeats by single forward primer driven PCR" @default.
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