FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2187083442> ?p ?o ?g. }

• W2187083442 endingPage "120" @default.
• W2187083442 startingPage "113" @default.
• W2187083442 abstract "Purpose: The purpose of this study was the separation of spermatogonial cells from prepubertal NMRI mouse testis by magnetic activating cell sorting( MACS ) method and assessment of FBS concentration on surviving these cells after cryopreservation. Materials and Methods: Testes from 6-days-old mouse removed and digested in two enzymatic mediums. first 8-9 min at medium containing collagenase, trypsin and DNase and second 10-12 min in medium containing collagenase, trypsin, DNase, hyalloronidase and EDTA and then the spermatogonial cells were isolated by ( MACS ) method. In the freezing step, the cells were frozen in three medium containing DMEM/F12 medium, 10% DMSO and 50%, 60% and 70% FBS serum ,named group I,II.III. Results: The viable cells obtained after enzymatic disaggregation were 91.66%±0.60% and after being isolated by MACS method were 95.25%±0.33%. The purity of the isolated cells was 93.79%±2.20%. The cells frozen in group I, II and III had 39.09%±0.15, 85.55%±6.98 and 90/29±1/38 % viability, respectively. Conclusion: according to the obtained results, the increase of temperature at digestion step reduces the time of testicular tissue disaggregation and consequently increase viable cells. Higher viable cells and purity can be attained by using of α6 integrin and magnetic beads. The results show that spermatogonial cells in freezing media containing 60% - 70% FBS have the highest viability after thawing." @default.
• W2187083442 created "2016-06-24" @default.
• W2187083442 creator A5013599048 @default.
• W2187083442 creator A5020633865 @default.
• W2187083442 creator A5023885204 @default.
• W2187083442 creator A5028016627 @default.
• W2187083442 creator A5087664599 @default.
• W2187083442 date "2009-01-01" @default.
• W2187083442 modified "2023-09-24" @default.
• W2187083442 title "The Effect of FBS Concentration on Cryopreservation of Isolated Spermatogonial Cells from Neonatal Mouse by MACS Method" @default.
• W2187083442 cites W1491511834 @default.
• W2187083442 cites W1917815519 @default.
• W2187083442 cites W1977109705 @default.
• W2187083442 cites W1993543573 @default.
• W2187083442 cites W2020663303 @default.
• W2187083442 cites W2040605613 @default.
• W2187083442 cites W2041706422 @default.
• W2187083442 cites W2045847970 @default.
• W2187083442 cites W2052441327 @default.
• W2187083442 cites W2068464983 @default.
• W2187083442 cites W2108908219 @default.
• W2187083442 cites W2110804359 @default.
• W2187083442 cites W2110920306 @default.
• W2187083442 cites W2113241496 @default.
• W2187083442 cites W2128984725 @default.
• W2187083442 cites W2131238404 @default.
• W2187083442 cites W2135328825 @default.
• W2187083442 cites W2141040878 @default.
• W2187083442 cites W2158842527 @default.
• W2187083442 cites W2160260477 @default.
• W2187083442 cites W2163846802 @default.
• W2187083442 cites W2165209578 @default.
• W2187083442 cites W2170748214 @default.
• W2187083442 cites W2398547988 @default.
• W2187083442 cites W2747310579 @default.
• W2187083442 hasPublicationYear "2009" @default.
• W2187083442 type Work @default.
• W2187083442 sameAs 2187083442 @default.
• W2187083442 citedByCount "1" @default.
• W2187083442 countsByYear W21870834422012 @default.
• W2187083442 crossrefType "journal-article" @default.
• W2187083442 hasAuthorship W2187083442A5013599048 @default.
• W2187083442 hasAuthorship W2187083442A5020633865 @default.
• W2187083442 hasAuthorship W2187083442A5023885204 @default.
• W2187083442 hasAuthorship W2187083442A5028016627 @default.
• W2187083442 hasAuthorship W2187083442A5087664599 @default.
• W2187083442 hasConcept C1491633281 @default.
• W2187083442 hasConcept C153911025 @default.
• W2187083442 hasConcept C16685009 @default.
• W2187083442 hasConcept C179933525 @default.
• W2187083442 hasConcept C181199279 @default.
• W2187083442 hasConcept C185592680 @default.
• W2187083442 hasConcept C196843134 @default.
• W2187083442 hasConcept C2778394429 @default.
• W2187083442 hasConcept C2778630268 @default.
• W2187083442 hasConcept C2780340462 @default.
• W2187083442 hasConcept C2781357212 @default.
• W2187083442 hasConcept C43617362 @default.
• W2187083442 hasConcept C53227056 @default.
• W2187083442 hasConcept C55493867 @default.
• W2187083442 hasConcept C71924100 @default.
• W2187083442 hasConcept C86803240 @default.
• W2187083442 hasConcept C95444343 @default.
• W2187083442 hasConceptScore W2187083442C1491633281 @default.
• W2187083442 hasConceptScore W2187083442C153911025 @default.
• W2187083442 hasConceptScore W2187083442C16685009 @default.
• W2187083442 hasConceptScore W2187083442C179933525 @default.
• W2187083442 hasConceptScore W2187083442C181199279 @default.
• W2187083442 hasConceptScore W2187083442C185592680 @default.
• W2187083442 hasConceptScore W2187083442C196843134 @default.
• W2187083442 hasConceptScore W2187083442C2778394429 @default.
• W2187083442 hasConceptScore W2187083442C2778630268 @default.
• W2187083442 hasConceptScore W2187083442C2780340462 @default.
• W2187083442 hasConceptScore W2187083442C2781357212 @default.
• W2187083442 hasConceptScore W2187083442C43617362 @default.
• W2187083442 hasConceptScore W2187083442C53227056 @default.
• W2187083442 hasConceptScore W2187083442C55493867 @default.
• W2187083442 hasConceptScore W2187083442C71924100 @default.
• W2187083442 hasConceptScore W2187083442C86803240 @default.
• W2187083442 hasConceptScore W2187083442C95444343 @default.
• W2187083442 hasIssue "2829" @default.
• W2187083442 hasLocation W21870834421 @default.
• W2187083442 hasOpenAccess W2187083442 @default.
• W2187083442 hasPrimaryLocation W21870834421 @default.
• W2187083442 hasRelatedWork W1941199801 @default.
• W2187083442 hasRelatedWork W1974101362 @default.
• W2187083442 hasRelatedWork W1974215053 @default.
• W2187083442 hasRelatedWork W2008269403 @default.
• W2187083442 hasRelatedWork W2025712217 @default.
• W2187083442 hasRelatedWork W2036491313 @default.
• W2187083442 hasRelatedWork W2065406334 @default.
• W2187083442 hasRelatedWork W2128586512 @default.
• W2187083442 hasRelatedWork W2343656797 @default.
• W2187083442 hasRelatedWork W2366312071 @default.
• W2187083442 hasRelatedWork W2366764195 @default.
• W2187083442 hasRelatedWork W2410918846 @default.
• W2187083442 hasRelatedWork W2413167091 @default.
• W2187083442 hasRelatedWork W2418305586 @default.

• next