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- W2187134538 abstract "3 Abstract: Mycoplasma bovine mastitis is a highly contagious disease that results in milk loss and culling of infected animals. Therefore the aim of this work was to focus on the diagnosis of masitic mycoplasma including California Mastitic Test (CMT), indirect ELISA (iELISA), Polymerase Chain Reaction (PCR) and bacteriological isolation. Beside emphathize the importance of Mycoplasma in subclinical mastitis. A total of 236 apparently normal quarter milk samples were examined for mastitis using CMT.The incidence of subclinical mastitis was 32.62 and 26.25% in cows and buffaloes, respectively. Examination of cows and buffaloes for Mycoplasma revealed 9.09 and 0% from subclinical mastitic animals and 14.73 and 14.29%, from clinically mastitic animals, respectively. Identification of the isolated Mycoplasma revealed more than one species. The most important was M. bovigenitalium (40.74% from the total isolates), then M.arginini (37.04%), M. bovis (18.52%) and. M.bovirihinis (3.70%). Examination of 46 and 6 udder tissue samples from buffaloes and cows for Mycoplasma revealed 0 and 3.33% M. arginini, respectively. A serological study of 45 milk samples by ELISA test revealed 22.22% positive samples to M. bovis and 37.77% samples to M.bovigenitalium while examination of 10 serum samples revealed 20% as M. bovis. Application of the PCR specific to M. bovis for the total isolates recovered from mastitic milk samples revealed 18.52% as M. bovis. Finally there were significant increase in the levels of lysozyme and nitric oxide in subclinical and clinical Mycoplasma positive samples compared with negative and normal samples." @default.
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- W2187134538 date "2008-01-01" @default.
- W2187134538 modified "2023-09-27" @default.
- W2187134538 title "Molecular Typing of Mycoplasma Species Recovered from Bovine Mastitis" @default.
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