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- W2187313158 abstract "2 Abstract: A new simple, rapid and accurate reverse phase high Performance liquid chromatography assay has been developed for the estimation of Streptomycin. The estimation of streptomycin was done by Hitachi D-2000 Elite HPLC system manager using gradient pump system. From two pumps (L-2130) A containing mobile phase Methanol was adjusted on 40% flow while the pump B containing Mobile phase buffer (Orthophosphoric acid + Triethylamine) on 60%. The flow rate was 1.0ml/min; the separation was done by using C-18 column and detected by UV-Visible Detector at 240 nm. The retention time was within 2.62-2.63 minutes. Standard curves were linear over the concentration range of 0.01 to 2 µg/L. The extraction recovery of streptomycin was withen 96 and 103%. The proposed method was found to be rapid accurate, repeatability and consistent. It was successfully applied for the analysis of the Streptomycin Sulphate. This was rapid from before reported method." @default.
- W2187313158 created "2016-06-24" @default.
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- W2187313158 date "2012-01-01" @default.
- W2187313158 modified "2023-09-26" @default.
- W2187313158 title "Simple and Rapid Method on High Performance Liquid Chromatography (HPLC) for Estimation of Streptomycin Sulphate" @default.
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