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- W2187339745 abstract ". Our study also demonstrated alterations to proteoglycan synthesis in cartilage explants cultured in normoxia compared to hypoxia. Reproducing hypoxic conditions in vitro may be important for studies of chondrocyte metabolic responses to injury, disease and potential treatments. This study was performed to test the hypothesis that chondrocyte viability and metabolism in threedimensional culture is enhanced by sustained hypoxia. METHODS: Cell culture. Articular chondrocytes grown in alginate beads provide a 3-dimensional (3D) environment for maintaining chondrocyte phenotype while allowing homogeneous oxygen levels throughout the culture. Alginate cultures also permit recovery of individual cells for study. Bovine articular chondrocytes (BAC) were obtained from knee cartilage of freshly slaughtered calves and prepared into alginate bead cultures at a density of 4x10 6" @default.
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- W2187339745 date "2005-01-01" @default.
- W2187339745 modified "2023-09-22" @default.
- W2187339745 title "SUSTAINED HYPOXIA ENHANCES CHONDROCYTE VIABILITY AND MATRIX SYNTHESIS IN 3-DIMENSIONAL CULTURE" @default.
- W2187339745 hasPublicationYear "2005" @default.
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