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- W2187437029 abstract "SUMMARY: The aim of this study was to evaluate the acute cell injury of excretion products present in culture filtrate from Shigella dysenteriae in both whole lower limb of chick embryo ex vivo and myoblasts cells developed in hanging-drop cultures in vitro.Three controls were defined: a) Tyrode’s solution b) brain-heart broth infusion (CCC) and c) supernatant not toxigenic of E.coli O157:H7. Shigella dysenteriae were culture for 24 hours and the excretion products were obtained after centrifugation of the culture.After 1h of treatment, the morphologic changes in limbs treated with raw filtrate were evaluated through histopathological exa minationof sections stained with hematoxylin-eosin (H&E-stain) and Gomo ri’s trichrome by image analysis techniques. Quantification of apo ptoticcells was measured by an enzyme-linked immunoassay TUNEL. The morphological feature of apoptosis were evaluated in culturemyoblasts. In contrast with controls, the longitudinal section on treated thigh of chick embryo limb-buds show atrophy muscle tissue,detachment of few fibers, 57,14% decrease in the number of cells, and loss of collagen substrate. Apoptotic index percent increase andmitotic index decrease in response to excretion products were observed, but were not significant. Membrane blebbing, vacuolation, smallaggregates of chromatin around the nucleus and loss of cell adhesion were observed. Culture filtrate from" @default.
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- W2187437029 date "2007-01-01" @default.
- W2187437029 modified "2023-09-28" @default.
- W2187437029 title "Excretion Products of Shigella dysenteriae and Apoptotic Cell Death on Chick Embryo Muscle Tissue Producto de Excreción de Shigella dysenteriae y la Muerte Celular por Apoptosis en Tejido Muscular de Embrión de Pollo" @default.
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