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- W2187443732 abstract "Use of probiotic bacteria in aquaculture has tremendous scope and the studies of the application of probiotics in aquaculture have a glorious future. The present study was aimed at the studies on the probiotic characters of bacteria which were isolated from shrimp cultured ponds. Initially 26 soil samples were collected from dried shrimp cultured ponds; bacteria were isolated by serial dilution. 10 samples were able to produce prominent colonies which were further subjected for repeated sub culturing for pure cultures on nutrient agar medium. Among 10 isolates six were Gram positive, and four of them were Gram negative. All isolates fermented glucose, three of them (HL1,HL2,HL6) fermented lactose, four (HL6,HL4,HL5andHL6) fermented xylose, six (HL1,HL2,HL4,HL8,HL9,HL10) gave positive result for catalase, four (HL1,HL2,HL4,HL6) showed positive result for urease test and four (HL1,HL2,HL3and HL4) gave positive result for nitrate reductase test. Probiotic bacteria such as lactic acid bacteria are mostly gram positive so only six gram positive strains were made pure culture on MRS agar which were further characterized for their probiotic nature. All the isolates survived under low pH (2and3) for 1hr and 6hrs. All isolated colonies survived under 0.3%, 0.5% and 0.1% oxgall treatments, HL1 (98±1.6cfu/ml) and HL5 (92±4.2cfu/ml) showed high survival rate under 0.3% concentration. All isolates survived in gastro intestinal juices for 4hrs, HL1 showed high survival rate 72±1.2cfu/ml. High cholesterol assimilated by HL5 (27±1.2cfu/ml). DNA was isolated from five strains except HL2 and DNA amplification of V2-V3 variable region for identification of Lactobacillus species." @default.
- W2187443732 created "2016-06-24" @default.
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- W2187443732 date "2014-01-01" @default.
- W2187443732 modified "2023-09-27" @default.
- W2187443732 title "Probiotic properties of bacterium isolated from shrimp cultured ponds" @default.
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