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- W2187624666 abstract "andnosocomial L.pneumophila infection havebeendescribed previously (6,15). Laboratory detection of Legionella infection remains problematic. Serological diagnosis, although highly specific, hasa sensitivity inthe70to80% range,andthese figures areconsiderably different foruse of single-antibody testsandfordetection ofantibody toLegionella species or serogroupsotherthanL.pneumophila serogroup 1.Theresults arealsohighly influenced bythekindof antigen (formolized or heated) usedforthediagnosis of seroconversion (7). Thesensitivity ofthedirect-fluorescence assay(DFA)forantigen detection remains highly variable and even insophisticated laboratories, suchas theStanford University Medical Center microbiology laboratory, thesensitivity ofDFA incomparison withculture was foundtobeonly30% (16). Theculture diagnosis remains thegoldstandard and probably continues tobethemostsensitive means ofdiagnosis whenitisperformed early inthecourse ofdisease. However, therecoveryoflegionellae byculture hasdifferent limits associated withthedegree ofspecimen contamination byother bacteria andthedifficulty presented fortheculture ofLegionella species otherthanL.pneumophila. Inaddition, this methodisnotwidely usedandwhenitis, itisdonepoorly. A 1989surveyofU.S.clinical microbiology laboratories found that32%oflaboratories were unable togrow a pureandheavy culture ofL.pneumophila (5). Forthese reasons,DNA amplification isan interesting alternative methodforbacterial detection. Amplification ofLegionella-specific" @default.
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- W2187624666 date "1994-01-01" @default.
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- W2187624666 title "Evaluation ofCommercial Amplification KitforDetection of Legionella pneumophila inClinical Specimens" @default.
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