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• W2187651262 abstract "Five experiments were conducted using explants from mature Hydrangea macrophylla plants, with the aim of developing an in vitro propagation protocol for production of multipurpose H. macrophylla plants. The results of exp. 1 indicated that the highest percentage of contaminationfree explants (100%) was obtained by using chlorox at 50% plus mercuric chloride (MC) at the concentration 0.2%. Data of exp. 2 indicated that using MS medium plus BA at the rate of 2 mg/l resulted in the highest number of shoots/explant. Increasing the number of subcultures significantly increased the number of shoot per explants, with the 3 rd subculture giving the highest value. Shoot length was significantly reduced by adding TDZ to the MS medium. MS medium plus BA or Kin at the rate of 2 mg/l were the most effective treatments in giving significant increases in shoot length. Culturing the explants on an MS medium supplemented with BA at 4 mg/l produced the highest number of leaves/explant. Results of full strength B5 medium was the best medium for causing significant increases in the number of shoot per explant. Increasing the number of subcultures significantly increased the number of shoot per explant. The longest shoots were obtained in the 3 rd subculture. Half strength B5 medium was the best treatment for producing the longest shoots in the three subcultures. Using full strength B5 medium led to production of the greatest number of leaves/explant. Results of exp. 4 showed that the greatest number of roots/shootlet recorded with 1/2 MS salt strength medium supplemented with 2 mg IBA/l and activated charcoal. The tallest plantlets were recorded when using MS medium supplemented with 3 mg/l IBA and activated charcoal. The highest number of leaves/shootlet was recorded by using MS medium supplemented with 2 and 3 mg/l IBA. Finally, result of exp. 5 showed that during adaptation, the tallest plants and the greatest number of leaves were observed when using peat moss plus sand (1:1 v/v), where the survival percentage was 100%." @default.
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• W2187651262 date "2007-01-01" @default.
• W2187651262 modified "2023-09-26" @default.
• W2187651262 title "In vitro propagation of Hydrangea macrophylla Thunb" @default.
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