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- W2187737265 abstract "Non-Governmental Organization Archaia (http://www.archaia.cz) carried out the rescue ar- chaeological research at Kněževes near Prague in 1998. Most of dating objects in Kněževes come from the period of Late and Final Bronze Age. The ap- proximately 3,000 years old set, which included 11 human remains from three settlement features, was collected for the study. First, gender was determined according to anthropological characteristics. Ancient DNA from bones was extracted by the phenol-chlo- roform procedure and N-phenacetylthiazolum bro- mide reagent. Polymerase chain reaction amplifica- tion of AMEL XY, part of amelogenin gene, with subsequent polyacrylamide gel electrophoresis and Short Tandem Repeats analysis followed. DNA pro- files of skeletal remains were obtained by the frag- mentation analysis of autosomal short tandem repeat markers. Genetic profiles showed us whether indi- viduals from Kněževes were in mutual relationship (parent - descendant). The congruence of results in sex determination supported reliability of genetic methods, which are suitable for sex determination of fragmental and subadult skeletal remains." @default.
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- W2187737265 date "2010-01-01" @default.
- W2187737265 modified "2023-09-27" @default.
- W2187737265 title "Original Article DNA Analysis of Ancient Skeletal Remains" @default.
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