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- W2187772012 abstract "3 Abstract: The comparative antimicrobial activity of tannins, saponins, alkaloids, essential oil, brown and yellow crystals separated from essential oil and thymoquinone, were evaluated, against a yeast (Saccharomyces cerevisiae), two molds (Penicillium notatum and Aspergillus niger), two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), five Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhimurium and Shigella flexneri) and an acid-fast bacterium (Mycobacterium phlei). Essential oil, its fractions and thymoquinone were the most active constituents against the tested microorganisms followed by alkaloids and saponins, respectively. On the contrary, tannins of N. sativa lacked any activity against all tested microorganisms. The tested Gram-positive bacteria were significantly (p < 0.0001) more sensitive than Gram-negative bacteria and Sac. cerevisiae was more sensitive than P. notatum and A. niger (p < 0.001) to the tested constituents. Concentrations as low as 4-16 µg/ml of the volatile oil or its fractions killed S. aureus and B. subtilis. The MICs and MBCs of the tested N. sativa constituents against both Gram-positive and Gram-negative bacteria in Muller-Hinton were 2 to 8 times higher than their values in M9 minimal medium suggesting an interaction of the tested constituents with the organic matters." @default.
- W2187772012 created "2016-06-24" @default.
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- W2187772012 date "2012-01-01" @default.
- W2187772012 modified "2023-09-26" @default.
- W2187772012 title "Comparative Antimicrobial Activity of Some Active Constituents of N. sativa L." @default.
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