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- W2187788484 abstract "Mesoporous silica nano-particles were used as the matrix to immobilize antibodies and enzymes which were used as reporter elements for biomolecular interaction. Luciferases of Jamaican click beetles and antibodies were immobilized on mesoporous silica nano-particles for immune bioluminescence detection of Staphylocuccus aureus enterotoxin B (SEB) and carcinoembryonic antigen (CEA). Fluorescent labeled mouse IgG and green fluorescent protein (GFP) were used to test and confirm the protein immobilization on the nanoparticles by fluorescent microscopic method. For the application of functionalized meso-porous bioluminescence nanoparticle in bio-molecular detection, two sets of nano-particle report elements were prepared: anti-SEB antibody with luciferase100 (red emission) and anti-CEA antibody with luciferase-99 (green emission). Antibody-antigen recognition was performed in 96 assay plates and on nitrocellulose membrane. In the presence of the target molecules, the enzymatic reaction on the nanoparticles generated light that was detected by using a CCD camera. The enzyme activity and antibody biological function on the nanoparticles were maintained more than 30 hrs in dry. Bioluminescence reaction of this new report element was also demonstrated on a CMOS color imaging chip based device." @default.
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- W2187788484 date "2008-06-01" @default.
- W2187788484 modified "2023-09-26" @default.
- W2187788484 title "Functionalized Mesoporous Silica Nano-Particle for Optical Bio-Detection" @default.
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