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- W2187799275 abstract "Therestriction enzymes AseI(ATTAAT), DraI(T'TTAAA), andSspI(AATATT)cuttheStreptomyces coelicolor A3(2) chromosome into 17,8,and25fragments separable bypulsed-field gelelectrophoresis (PFGE). Thesumsoftheir lengths indicated that thechromosome consists ofabout 8MbofDNA,some75%morethan thatofEscherichia coli K-12. A physical mapofthechromosome wasconstructed forAseIandDral, using single anddouble digests, linking clones, cross-hybridization ofrestriction fragments, andlocations of genetically mappedgenes, insertion sequences, prophages, andtheintegrated SCP1andSLP1plasmids onthe physical map.Thephysical mapwasaligned with thepreviously established genetic map,revealing that thetwo long opposite quadrants ofthegenetic mapthat arealmost devoid ofmarkers (the silent regions at3o'clock and9o'clock) areindeed physically long rather thanbeing hotspots forgenetic exchange. Theymusttherefore contain longstretches ofDNA different infunction fromtheremainder ofthegenome. Consistent withthis conclusion arethelocations ofsignificant deletions inbothofthesilent regions. Ofthese, a40-kbdeletion in the9o'clock region accompanied orfollowed integration oftheSCP1linear plasmid toproduce theNFfertility state. PFGEanalysis ofStreptomyces lividans 66,aclose relative ofS.coelicolor A3(2), washampered bythe previously described susceptibility ofits DNA todegradation during electrophoresis. However, ZX7,amutant derivative ofS.lividans lacking theDNA modification responsible forthis degradation, yielded goodPFGE preparations. Notmorethan7ofthe17S.coelicolorAsel fragments could beshared bytheS.lividans strain." @default.
- W2187799275 created "2016-06-24" @default.
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- W2187799275 date "1992-01-01" @default.
- W2187799275 modified "2023-09-27" @default.
- W2187799275 title "A Combined Genetic andPhysical MapoftheStreptomyces coelicolor A3(2)Chromosome" @default.
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