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• W2187805989 abstract "Nitrovasodilators-sodium nitroprusside (SNP; 10 −9 -10 −4 M) and 3-morpholino-sydnonimine (SIN-1; 10 −9 -10 −4 M) produced concentration-dependent relaxation of the fourth generation sheep pulmonary artery, preconstricted with 5hydroxytryptamine (1 μM). Oxidizing agents [oxidized glutathione (GSSG, 1 mM) and CuSO4 (5 and 20 μM)] and reducing agents [dithiothreitol (DTT, 0.1 mM), ascorbic acid (1 mM) and reduced glutathione (GSH, 1 mM)] caused opposite effects on nitric oxide (NO)-induced vasodilation in the artery. Ascorbic acid and GSH potentiated the NO responses, while GSSG and CuSO4 inhibited relaxation caused by the nitrovasodilators. DTT, however, reduced the relaxant potency and efficacy of SNP and SIN-1. Pretreatment of the pulmonary artery strips with DTT (0.1 mM) inhibited SNP (10 µM)-induced Na +- K +ATPase activity, while ascorbic acid (1 mM) and GSH (1 mM) had no effect either on basal or SNP (10 µM)-stimulated 86 Rb uptake, an index of Na +- K +- ATPase activity, in ovine pulmonary artery. The results suggest that reducing agents like ascorbic acid may have beneficial effect in improving the vascular function under oxidative stress." @default.
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• W2187805989 date "2006-12-01" @default.
• W2187805989 modified "2023-09-27" @default.
• W2187805989 title "Effects of oxidizing and reducing agents on ovine pulmonary artery responses to nitric oxide donors, sodium nitroprusside and 3-morpholino-sydnonimine." @default.
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